Foto PD Dr. Ralph Bertram Assoc. Prof. Ralph Bertram

Forschungsreferent
Klinikum Nürnberg Medical School GmbH und Paracelsus Medizinische Privatuniversität

Tel: 0911 398-6754
E-Mail: ralph.bertram@klinikum-nuernberg.de
Fax: 0911 398-6751

Prof.-Ernst-Nathan-Str. 1
90419 Nürnberg
Deutschland
Haus: 38, EG

Qualifikationen

Diplom in Biologie, Dr. rer. nat., Habilitation für das Fach Mikrobiologie, Associate Professur in experimenteller Mikrobiologie der PMU Salzburg

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Mitgliedschaften und Engagement

 Gutachtertätigkeiten für Forschungsförderinstitutionen

 Gutachtertätigkeiten für Fachjournale

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Publikationen

Übersicht in PubMed

 

2017


Schmitter, S., Fieseler, L., Klumpp, J., Bertram, R. and Loessner M. J.
TetR-dependent gene regulation in intracellular Listeria monocytogenes demonstrates the spatiotemporal surface distribution of ActA
Mol Microbiol. 2017 Aug;105(3):413-425. doi: 10.1111/mmi.13706. Epub 2017 May 25.


Xu, T., Wu, Y., Lin, Z., Bertram, R., Götz, F., Zhang, Y. and Qu, D.
Identification of Genes Controlled by the Essential YycFG Two-Component System Reveals a Role for Biofilm Modulation in Staphylococcus epidermidis 
Front Microbiol. 2017 Apr 26;8:724. doi: 10.3389/fmicb.2017.00724. eCollection 2017.


Meiers, M., Laux, A., Eichinger, D., Sexauer, A., Marx, P., Bertram, R. and Brückner, R.
A tetracycline-inducible integrative expression system for Streptococcus pneumoniae
FEMS Microbiol Lett. 2017 Mar 1;364(5). doi: 10.1093/femsle/fnx044.

 

2016


Schuster, C. F. and Bertram, R.
Toxin-Antitoxin Systems of Staphylococcus aureus
Toxins (Basel). 2016 May 5;8(5). pii: E140. doi: 10.3390/toxins8050140


Bertram, R.
Persisters run out of fuel
Nature Microbiol. 2016 (1): doi: 10.1038/NMICROBIOL.2016.60.


Prax, M., Mechler, L., Weidenmaier, C. and Bertram, R.
Glucose Augments Killing Efficiency of Daptomycin Challenged Staphylococcus aureus Persisters
PLoS One. 2016 Mar 9;11(3):e0150907. doi: 10.1371/journal.pone.0150907.


Mechler, L., Bonetti, E. J., Reichert, S., Flötenmeyer, M., Schrenzel, J., Bertram, R., François, P. and Götz, F.
Daptomycin tolerance in the Staphylococcus aureus pitA6 mutant is due to upregulation of the dlt-operon
Antimicrob Agents Chemother. 2016 Feb 16. pii: AAC.03022-15.


Bertram, R.
Complementation Plasmids, Inducible Gene-Expression Systems, and Reporters for Staphylococci
Methods Mol Biol. 2016;1373:25-32. doi: 10.1007/7651_2014_181.

 

2015


Mechler, L., Herbig, A., Paprotka, K., Fraunholz, M., Nieselt, K. and Bertram R.
A novel point mutation promotes growth phase-dependent daptomycin tolerance in Staphylococcus aureus
Antimicrob Agents Chemother. 2015 Jun 22. pii: AAC.00643-15.


Schuster, C. F., Mechler, L., Nolle, N., Krismer, B., Zelder, M. E., Götz, F. and Bertram, R. 
The MazEF Toxin-Antitoxin System Alters the β-Lactam Susceptibility of Staphylococcus aureus
PLoS One. 2015 May 12;10(5):e0126118. doi: 10.1371/journal.pone.0126118. eCollection 2015.

 

2014


Schuster, C. F. and Bertram R.
Fluorescence Based Primer Extension Technique to Determine Transcriptional Starting Points and Cleavage Sites of RNases In Vivo
J Vis Exp. 2014 Oct 31;(92). doi: 10.3791/52134.


Prax, M. and Bertram R. 
Metabolic aspects of bacterial persisters
Front Cell Infect Microbiol.2014 Oct 22;4:148. eCollection 2014.


Koczula, A., Willenborg, J., Bertram, R., Takamatsu, D., Valentin-Weigand, P. and Goethe, R.
Establishment of a Cre recombinase based mutagenesis protocol for markerless gene deletion in Streptococcus suis
J Microbiol Methods. 2014 Dec;107:80-3. doi: 10.1016/j.mimet.2014.09.007. Epub 2014 Oct 1


Lechner, S., Prax, M., Lange, B., Huber, C., Eisenreich, W., Herbig, A., Nieselt, K. and Bertram R.
Metabolic and transcriptional activities of Staphylococcus aureus challenged with high-doses of daptomycin
Int J Med Microbiol. 2014 May 28. pii: S1438-4221(14)00059-9. doi: 10.1016/j.ijmm.2014.05.008.


Willenborg, J., Willms, D., Bertram, R., Goethe, R. and Valentin-Weigand, P. 
Characterization of multidrug tolerant persister cells in Streptococcus suis
BMC Microbiol. 2014 May 12;14(1):120. doi: 10.1186/1471-2180-14-120.


Bertram, R. and Schuster C. F.
Post-transcriptional regulation of gene expression in bacterial pathogens by toxin-antitoxin systems
Front Cell Infect Microbiol. 2014 Jan 29;4:6. doi: 10.3389/fcimb.2014.00006. eCollection 2014.

 

2013


Hartmann, T., Zhang, B., Baronian, G., Schulthess, B., Homerova, D., Grubmüller, S., Kutzner, E., Gaupp, R., Bertram, R., Powers, R., Eisenreich, W., Kormanec, J., Herrmann, M., Molle, V., Somerville, G. A. and Bischoff, M.
Catabolite control protein E (CcpE) is a LysR-type transcriptional regulator of TCA cycle activity in Staphylococcus aureus
J Biol Chem. 2013 Dec 13;288(50):36116-36128


Yu, W., Leibig, M., Schäfer, T., Bertram, R., Ohlsen, K. and Götz, F.
Mevalonate auxotrophic mutant of Staphylococcus aureus can adapt to mevalonate depletion
Antimicrob Agents Chemother. 2013 Nov;57(11):5710-5713


Nolle, N., Schuster, C. F. and Bertram, R. 
Two paralogous yefM-yoeB loci from Staphylococcus equorum encode functional toxin-antitoxin systems
Microbiology 2013 Aug;159(Pt 8): 1575-1585


Prax, M., Lee, C. Y. and Bertram, R. 
An update on the molecular genetics toolbox for staphylococci 
Microbiology 2013 Mar;159(Pt 3):421-434


Schuster, C. F. and Bertram, R. 
Toxin-antitoxin systems are ubiquitous and versatile modulators of prokaryotic cell fate
FEMS Microbiol Lett. 2013 Mar;340(2):73-85


Lechner, S., Patra, P., Klumpp, S. and Bertram, R. 
Interplay between population dynamics and and drug tolerance of Staphylococcus aureus persister cells
J Mol Microbiol and Biotechnol. 2013 Jan 25;22(6):381-391


Schuster, C. F., Park J. H., Prax M., Herbig A., Nieselt K., Rosenstein R., Inouye, M. and Bertram, R. 
Characterization of a mazEF toxin-antitoxin homologue from Staphylococcus equorum
J Bacteriol. 2013 Jan;195(1):115-25

 

2012 und älter


Lechner, S., Lewis, K. and Bertram, R. 
Staphylococcus aureus persisters tolerant to bactericidal antibiotics
J Mol Microbiol and Biotechnol. 2012;22(4):235-244


Gauger, T., Weihs, F., Mayer, S., Krismer, B., Liese, J., Kull, M. and Bertram, R. 
Intracellular monitoring oftarget protein production in Staphylococcus aureus by peptide tag-induced reporter fluorescence
Microb Biotechnol 2012 Jan;5(1):129-134


Helle, L., Kull, M., Mayer, S., Marincola, G., Zelder, M.-E., Goerke, C., Wolz, C. and Bertram, R.
Vectors for improved Tet repressor -dependent gradual gene induction or silencing in Staphylococcus aureus
Microbiology 2011 Dec;157(Pt 12):3314-3323


Bertram, R.*, Rigali, S.*, Wood, N., Lulko, A. T., Kuipers O. P. and Titgemeyer, F. 
Regulon of the N-Acetylglucosamine Utilization Regulator (NagR) in Bacillus subtilis
J Bacteriol 2011 Jul;193(14):3525-3536 (* equally contributing authors)


Stary, E., Gaupp, R., Lechner, S., Leibig, M., Tichy, E., Kolb, M. and Bertram, R. 
New architectures for Tet-ON and Tet-OFF regulation in Staphylococcus aureus
Appl Environ Microbiol. 2010 Feb;76(3):680-687


Biswas, L., Biswas, R., Schlag, M., Bertram, R. and Götz, F.
Small-colony variant selection as a survival strategy for Staphylococcus aureus in the presence of Pseudomonas aeruginosa
Appl Environ Microbiol. 2009 Nov;75(21):6910-6912


Bertram, R., Kolb, M. and Hillen, W.
In vivo activation of Tetracycline repressor by Cre/lox mediated gene assembly
J Mol Microbiol and Biotechnol 17(3):136-145


Resch, M., Roth H. M., Kottmair, M., Sevvana, M., Bertram, R., Titgemeyer, F. and Muller, Y. A.
Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of YvoA from Bacillus subtilis
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Apr;65(4): 410-414 


Leibig, M., Krismer, B., Kolb, M., Friede, A., Götz, F., and Bertram, R.
Marker removal in staphylococci via Cre recombinase and different lox sites
Appl Env Microbiol 2008 Mar;74(5):1316-1323


Bertram, R. and Hillen, W. 
The application of Tet repressor in prokaryotic gene regulation and expression 
Microb Biotechnol 2008 Jan;1(1):2-16


Kamionka, A., Majewski, M., Roth, K., Bertram, R., Kraft, C. and Hillen, W.
Induction of tetracycline repressor requires the binding of two inducers
Nucleic Acids Res 2006;34(14):3834-3841


Köstner, M., Schmidt, B., Bertram, R. and Hillen, W.
Generating tetracycline inducible auxotrophy in Escherichia coli and Salmonella enterica serovar Typhimurium by using an insertion element and a hyperactive transposase
Appl Env Microbiol 2006 Jul;72(7):4717-4725


Bertram, R., Wünsche, A., Sprehe, M. and Hillen, W. 
Regulated Expression of HPrK/P does not affect Carbon Catabolite Repression of the xyn Operon and of rocG in Bacillus subtilis
FEMS Microbiol Lett. 2006 Jun;259(1):147-152


Bertram, R., Köstner, M. Müller, J., Vazquez Ramos, J. and Hillen, W.
Integrative elements for Bacillus subtilis yielding tetracycline-dependent growth phenotypes
Nucleic Acids Res 2005 Oct;33(18):e153


Henßler, E. M., Bertram, R., Wisshak, S. and Hillen, W. 
Tet repressor mutants with altered effector binding and allostery
FEBS Journal 2005 Sep;272(17):4487-4496


Bertram, R., Kraft, C., Wisshak, S., Müller, J., Scholz, O. and Hillen, W.
Phenotypes of combined Tet repressor mutants for effector and operator recognition and allostery
J Mol Microbiol and Biotechnol 2004;8(2):104-110


Kamionka, A., Bertram, R. and Hillen, W.
Tetracycline dependent conditional gene knockout in Bacillus subtilis
Appl Env Microbiol 2005 Feb;71(2):728-733


Scholz, O., Henßler, E. M., Bail, J., Schubert, P., Bogdanska-Urbaniak, J., Sopp, S., Reich, M., Wisshak, S., Köstner, M., Bertram, R. and Hillen, W.
Activity reversal of Tet repressor by single amino acid exchanges
Mol Microbiol 2004 Aug;53(3):777-789


Bertram, R., Schlicht, M., Mahr, K., Nothaft, H., Saier, M. H. jr. and Titgemeyer F.
In silico and transcriptional analysis of carbohydrate uptake systems of Streptomyces coelicolor A3(2)
J Bacteriol 2004 Mar;186(5):1362-1373.

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